Clostridium ultunense DSM 10521 genome sequencing. [Clostridium] ultunense DSM 10521

Clostridium ultunense DSM 10521. Clostridium ultunense DSM 10521 was purchased from DSMZ for genome sequencing, however 16S rDNA clone library constructed from this strain indicated that this strain was contaminated by a unknown strain related to Aminobacterium colombiense DSM 12261 (16S rDNA similarity>98%) and this result was confirmed by DSMZ. Clostridium ultunense DSM 10521 was enriched to about 70% abundance in the mix culture, which was used for DNA extraction and then 454 GS FLX Sequencing. Clostridium ultunense DSM 10521T was reported as a typical mesophilic syntrophic acetate oxidizer. In our biogas metagenome project (ID: 50503), a set of 16S rDNA sequences were closely matched to this species phylogenetically. Therefore, this strain was selected to sequence as a reference genome for this project.