16s rRNA Honey bee worker hindgut microbiome

The study examines the initial acquisition and subsequent dynamics of the honey bee worker hindgut bacterial microbiome, and the effects of antibiotic (tylosin) treatment on these dynamics. This study used a colony-level treatment design to preserve natural and field-realistic worker conditions including diet, exposures to nestmates, and access to the foraging environment. Tylosin was applied to whole colonies in a field-realistic manner and bees were sampled from treated and control colonies for comparison. Age-controlled workers were sampled at eight age points to provide a broad overview of microbiome development from pupal emergence to the onset of foraging, including age (in days) 1, 2, 3, 4, 7, 14, 17, and 21.本研究聚焦于工蜂后肠细菌微生物组的初始定植与后续动态变化，以及抗生素泰乐菌素（tylosin）干预对该动态过程的影响。本研究采用蜂群水平处理实验设计，以维持工蜂所处的自然且符合田间实际的生存条件，涵盖其饮食、与巢伴的接触以及觅食环境的可及性。研究以符合田间实际的方式将泰乐菌素施用于完整蜂群，并从处理组与对照组蜂群中采集工蜂样本以用于对比分析。研究人员在8个日龄节点对日龄受控的工蜂进行采样，以全面覆盖从蛹羽化至觅食行为启动阶段的微生物组发育过程，采样日龄分别为1、2、3、4、7、14、17和21日龄。