Rat VTA single nuclei RNA sequencing

We performed single nuclei transcriptomics of ventral tegmental area samples from rats sacrificed one hour after a single saline injection and profiled the cell types and their receptor composition. Overall design: Fresh frozen postmortem rat ventral tegmental area samples were used to generate single nuclei suspensions and the nuclei were processed for the 10x Genomics 3' gene expression assay v3.1.