TABLE 7 in Comparing whole-genome shotgun sequencing and DNA metabarcoding approaches for species identification and quantification of pollen species mixtures

TABLE 7 Binomial mixed-effects model to assess if species richness, species relatedness, and pollen grain proportion have a significant effect on the proportion of false-positive sequencing reads identified in a pollen mixture through WGS analyzed with kraken Measure of mixture complexityTaxonomic levelp -valuenSpecies richnessSpecies.895764017Genus.381216017Taxonomic relatednessSpecies.541257817Genus.098472017Pollen grain proportionSpeciesN/A a17GenusN/A17 a Model did not converge.表7 用于评估物种丰富度、物种亲缘关系及花粉粒比例是否对经全基因组测序（Whole Genome Sequencing, WGS）并采用Kraken分析的花粉混合物中鉴定所得假阳性测序读段占比产生显著影响的二项混合效应模型 混合物复杂度衡量指标 分类学层级 p值 样本量(n) 物种丰富度 物种 0.895764017 17 物种丰富度 属 0.381216017 17 分类学相关性 物种 0.541257817 17 分类学相关性 属 0.098472017 17 花粉粒比例 物种 N/A a 17 花粉粒比例 属 N/A 17 a 模型未收敛。