PLA Figure 2 Positive Series

The second set of images provided is a set of photomicrographs acquired on methanol fixed T47D breast cancer cells and subjected to proximity ligation assays using antibodies to detect interactions between CDK2 and Cyclin E1 and counter stained with ToPro3/DAPI (nuclei) and Phalloidin (cytoplasm)本次提供的第二组图像为显微照片（photomicrographs），采集自经甲醇固定的T47D乳腺癌细胞。该组样本通过使用靶向细胞周期蛋白依赖性激酶2（CDK2）与细胞周期蛋白E1（Cyclin E1）相互作用的抗体进行邻近连接分析法（proximity ligation assay）检测，并以ToPro3/DAPI（标记细胞核）与鬼笔环肽（Phalloidin）完成复染色。