Table 2 in Biosynthesis of natural and novel C-glycosylflavones utilising recombinant Oryza sativa C-glycosyltransferase (OsCGT) and Desmodium incanum root proteins

Table 2 Generation of novel mono- C -glucosylfavones from 2-hydroxyflavanones and UDP-glucose by the Oryza sativa OsCGT. R3 0R4 0R5 08- C -Glucoside %6- C -Glucoside %3hO-CH2––OH5h; 44*6h; 52*3iOHHOH5i; 476i; 433jOHHH5j; 146j; 253kHOEtH5k; 346k; 383lHO(CH2)3OHH5l; 176l; 273mHO(CH2)6OHH5m; 06m; 03nHOPhH5n; 06n; 03oHMeH5o; 25*6o; 33*3pHEtH5p; 33 *6p; 41 *3qHt -BuH5q; 06q; 03rFOHH5r; 42 *6r; 56*3sHCF3H5s; 346s; 423tHFH5t; 45*6t; 47*3uHBrH5u; 06u; 03vBrOHH5v; 46v; 43wHClH4w; 06w; 03xClOHH5x; 06x; 0 * Products characterised by NMR spectroscopy.表2 利用水稻（Oryza sativa）OsCGT酶，以2-羟基黄烷酮与尿苷二磷酸葡萄糖（UDP-glucose）为底物合成新型单-C-葡萄糖基黄酮类化合物 R3 R4 R5 8-C-葡萄糖苷占比/% 6-C-葡萄糖苷占比/% 3h O-CH₂OH H 5h; 44* 6h; 52* 3i OH H H 5i; 47 6i; 43 3j OH H H 5j; 14 6j; 25 3k OEt H H 5k; 34 6k; 38 3l O(CH₂)₃OH H H 5l; 17 6l; 27 3m O(CH₂)₆OH H H 5m; 0 6m; 0 3n OPh H H 5n; 0 6n; 0 3o OMe H H 5o; 25* 6o; 33* 3p H Et H 5p; 33* 6p; 41* 3q H t-Bu H 5q; 0 6q; 0 3r F H H 5r; 42* 6r; 56* 3s CF₃ H H 5s; 34 6s; 42 3t H F H 5t; 45* 6t; 47* 3u H Br H 5u; 0 6u; 0 3v Br OH H 5v; 46 6v; 43 3w H Cl H 4w; 0 6w; 0 3x Cl OH H 5x; 0 6x; 0 * 产物经核磁共振波谱（NMR spectroscopy）表征