Data for: Effect of exogenous treatment with zaxinone and its mimics on rice root microbiota across different growth stages

This databse contains data generated in the following study:Mazzarella, T., Chialva, M., de Souza, L.P. et al. Effect of exogenous treatment with zaxinone and its mimics on rice root microbiota across different growth stages. Sci Rep 14, 31374 (2024). https://doi.org/10.1038/s41598-024-82833-616S_data.RData / ITS2_data.RData: R environments containing source meta-barcoding data for 16S (Prokaryotes) and ITS2 (Fungi) dataset, respectively. Each environment contains two phyloseq objects:feature_phylos: raw count matrix (ASVs table) with contaminants already removed using decontam package using blank samplesfeature_phylos_filtered_DESeq: ASVs table normalized using DESeq2 normalized counts (median of ratios) on raw counts filtered by abundance with HTSfilter R package.ASVphylo_DA.nwk: phylogenetic tree of differentially abundant ASV in Zax and MiZax treatments versus the acetone control.ASV.MSA_DA.fna: sequence aligment used to reconstruct phylogeny of differentially abundant ASV in Zax and MiZax treatments versus the acetone control.本数据库包含下述研究产生的实验数据：Mazzarella T.、Chialva M.、de Souza L.P. 等学者的研究论文《外源性玉米新酮（zaxinone）及其模拟物处理对不同生育期水稻根系微生物群的影响》，发表于《Sci Rep》14卷，31374 (2024)，DOI链接：https://doi.org/10.1038/s41598-024-82833-6。 16S_data.RData / ITS2_data.RData：分别存储16S（原核生物）和ITS2（真菌）数据集的宏条形码测序原始数据的R环境文件，每个R环境包含两个phyloseq对象： feature_phylos：已通过decontam R软件包结合空白样本去除污染物的原始计数矩阵（扩增子序列变异（ASVs, Amplicon Sequence Variants）表） feature_phylos_filtered_DESeq：经HTSfilter R软件包按丰度过滤原始计数后，使用DESeq2的比率中位数法进行标准化处理的ASVs表。 ASVphylo_DA.nwk：对比丙酮对照组，玉米新酮（zaxinone）及其模拟物（MiZax）处理组中差异丰度ASVs的系统发育树文件。 ASV.MSA_DA.fna：用于重建上述差异丰度ASVs系统发育树的序列比对文件。