Identification of VdCYC8-complemented mutants in Verticillium dahliae by PCR identification and VdCYC8 relative expression analysis.
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https://figshare.com/articles/dataset/_Identification_of_VdCYC8_complemented_mutants_in_Verticillium_dahliae_by_PCR_identification_and_VdCYC8_relative_expression_analysis_/1618176
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A) Confirmation of the presence of the wild-type complemented VdCYC8 fragment. B) Confirmation of the presence of the chlorimuron cassette in the ΔCYC8-C strains. Lanes 1–15 indicate PCR results using primer Sul-F/R and DNA template obtained from the different complemented mutant strains, respectively; Lane 16 represents T286. C) Relative expression analysis of VdCYC8 using primer CYC8RT-F/R. *Indicates a significant difference between mutant and the wild-type strain Vd080 (P
创建时间：
2016-02-22
