Identification of VdCYC8-complemented mutants in Verticillium dahliae by PCR identification and VdCYC8 relative expression analysis.
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A) Confirmation of the presence of the wild-type complemented VdCYC8 fragment. B) Confirmation of the presence of the chlorimuron cassette in the ΔCYC8-C strains. Lanes 1–15 indicate PCR results using primer Sul-F/R and DNA template obtained from the different complemented mutant strains, respectively; Lane 16 represents T286. C) Relative expression analysis of VdCYC8 using primer CYC8RT-F/R. *Indicates a significant difference between mutant and the wild-type strain Vd080 (P
A) 野生型互补VdCYC8片段的存在性验证。 B) ΔCYC8-C菌株中氯嘧磺隆抗性盒（chlorimuron cassette）的存在性验证。泳道1至15分别为使用引物对Sul-F/R以及从不同互补突变菌株中提取的DNA模板所得的聚合酶链式反应（PCR）结果；泳道16代表菌株T286。 C) 采用引物对CYC8RT-F/R开展的VdCYC8相对表达量分析。*表示突变菌株与野生型菌株Vd080之间存在显著差异（P
创建时间：
2016-02-22
