Transcriptome analysis of Arabidopsis thaliana roots in response to high nickel

To gain further insights into a larger number of processes potentially altered by high nickel (Ni), we performed a transcriptional profiling of whole roots of Arabidopsis thaliana accession Columbia-0 (Col-0) exposed to 100 µM nickel, a concentration that induces slight chlorosis and intermediate inhibition of root and shoot growth. Gene expression of whole roots of Arabidopsis thaliana (accession Col) after transferring seven-day-old seedlings were transferred to solid half-strength MS agar media containing no added nickel (control) or 100 µM NiSO4 (+nickel) for 4 days. Four biological replicates were used.