Nucleoside quantification in the plasma of G2019S LRRK2 knockin mice

This Zenodo deposit contains a publicly available description of the Dataset: Title: "Nucleoside quantification in the plasma of G2019S LRRK2 knockin mice". Description: G2019S LRRK2 knockin mice and their wild-type littermates (3-5 months old) were anesthetized with an isoflurane vaporizer, and whole blood was collected from cardiac puncture. 50 μL plasma from each mouse was split and processed using the Bligh-Dyer method. Absolute nucleoside quantitation was accomplished by running an external calibration curve with the samples. The stock mix contained Deoxyadenosine (dA), Deoxycytidine (dC), Deoxyguanosine (dG), Deoxythymidine (dT), and Deoxyuridine (dU). Targeted metabolomics peak picking and integration were conducted in Skyline (v25.1) using accurate mass MS1, MS2 fragmentation pattern matching, and retention time derived from analytical standards run through each chromatography method. Raw data files for all samples of a given experiment were imported and metabolite peaks were auto-integrated based standard verified m/z, precursor adducts, and retention times for all metabolites of interest. Each experimental group contained biological replicates (n =8-9 per group). This dataset is made available to researchers via the ASAP CRN Cloud: cloud.parkinsonsroadmap.org. Instructions for how to request access can be found in the User Manual. This research was funded by the Aligning Science Across Parkinson's Collaborative Research Network (ASAP CRN), through the Michael J. Fox Foundation for Parkinson's Research (MJFF). This Zenodo deposit was created by the ASAP CRN Cloud staff on behalf of the dataset authors. It provides a citable reference for a CRN Cloud Dataset本Zenodo存档包含某公开可用数据集的说明： 标题：《G2019S LRRK2敲入小鼠血浆核苷定量分析》 实验描述：选取3至5月龄的G2019S LRRK2敲入小鼠及其野生型同窝仔鼠，使用异氟烷蒸发器进行麻醉，通过心脏穿刺采集全血。每份小鼠的50μL血浆均分后采用布莱-戴尔法（Bligh-Dyer method）进行前处理。通过外标校准曲线实现绝对核苷定量，校准储备液包含脱氧腺苷（Deoxyadenosine, dA）、脱氧胞苷（Deoxycytidine, dC）、脱氧鸟苷（Deoxyguanosine, dG）、脱氧胸苷（Deoxythymidine, dT）及脱氧尿苷（Deoxyuridine, dU）。靶向代谢组学的峰识别与积分采用Skyline（v25.1）软件完成，依托精确质量数MS1、MS2碎裂模式匹配，以及通过各色谱方法分析的分析标准品得到的保留时间进行匹配。将单次实验所有样本的原始数据文件导入后，基于经标准验证的m/z值、前体加合物及目标代谢物的保留时间，自动积分代谢物峰。各实验组均设置生物学重复（每组n=8~9）。 本数据集通过ASAP CRN云平台（ASAP CRN Cloud，cloud.parkinsonsroadmap.org）向研究人员开放，访问申请流程详见《用户手册》。 本研究由帕金森病研究对齐科学协作网络（Aligning Science Across Parkinson's Collaborative Research Network, ASAP CRN）通过迈克尔·J·福克斯帕金森病研究基金会（Michael J. Fox Foundation for Parkinson's Research, MJFF）资助。 本Zenodo存档由ASAP CRN云平台工作人员代表数据集作者创建，可为CRN云平台数据集提供可引用的参考文献。