Z. Xu, H. Ogawa, P. Vagnarelli, J.H. Bergmann, D.F. Hudson, S. Ruchard, T. Fukagawa, W.C. Earnshaw, K. Samejima (2011) CIL:12168, Gallus gallus gallus, memory B cell. CIL. Dataset

Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Aurora B localizes to centromeres in early mitosis, but fails to transfer to the spindle midzone during late mitosis in wild type clone 18 cells grown in the Aurora B inhibitor ZM447439. Cells were stained for Aurora B (green), plus alpha-tubulin (red) and DAPI for DNA (blue). See Fig 6E panel 9 in Xu et al. 2009 J Cell Biol 187, 637-653.

DT40鸡B淋巴细胞系细胞在有丝分裂阶段的细胞。使用Deltavision系统搭载100x/1.4NA planapo物镜进行的宽场免疫荧光图像的Z系列扫描，并通过约束迭代算法（Applied Precision）进行去卷积处理。展示的是中心Z切片。在早期有丝分裂中，Aurora B定位于着丝粒，但在野生型克隆18细胞中，在Aurora B抑制剂ZM447439的作用下，Aurora B未能转移至后期有丝分裂的纺锤体中区。细胞经Aurora B（绿色）、α-微管蛋白（红色）和DAPI（蓝色）染色。见图6E第9面板，Xu等，2009年，《细胞生物学杂志》第187卷，第637-653页。