Transcriptomic analysis of KDM4B-mediated genes in AGS cells

KDM4B, an important epigenetic regulator of cell proliferation, metastasis and genome stability, is often overexpressed in gastric cancer. Notably, elevated expression of KDM4B is associated with a poor clinical outcome. A global transcriptomic analysis between KDM4B control and KDM4B-knockdown AGS cells without or with Helicobacter pylori challenge reveals differentially expressed genes involved in response to virus, multi-organism process, and response to stimulus, suggesting KDM4B as an inducible epigenetic factor under H. pylori challenge. KDM4B control and knockdown cell lines were established in AGS cells based on the lentivirus system: pLKO (control) and shKDM4B (shRNA-specific RNA) lines. Global expression analysis was performed in AGS cells (pLKO vs. KDM4B-knockdown) uninfected or infected by H. pylori at an moi of 50 for 6 h. Microarray analysis was conducted by the National Health Research Institutes, using Affymetrix GeneChip Human Gene 2.0 ST Array (Affymetrix, Santa Clara, California, USA).