Additional file 1 of The sperm epigenome does not display recurrent epimutations in patients with severely impaired spermatogenesis

Additional file 1: Table S1 Clinical parameters. Table S2.MEST, H19, XIST and DDX4 methylation values obtained by DBS for the 133 sperm samples. Table S3. WGBS statistics. Table S4. WGBS methylation values for the 50 imprinting controls regions. Table S5. Annotations and WGBS methylation values for the 2764 soma-sperm DMRs. Table S6. WGBS methylation values for the Luján et al. 2019 DMRs. Table S7. Annotations and WGBS methylation values for the NC-NMO DMRs. Table S8. Primers for generating amplicons for targeted bisulfite sequencing. Table S9. Average WGBS methylation values (minimum coverage 5) for the subset of CpGs analysed by DBS in 17 NC-NMO DMR. Table S10. Statistical comparison of WGBS and WGBS CpG subset values (WGBS_s5). Table S11. Average DBS methylation values in 17 NC-NMO DMR. Table S12. Statistical tests concerning NC-NMO DMRs. Table S13. Methylation values of the 14 CpG sites in the H19 locus. Table S14. CpG3 methylation values in the T and G alleles in the TG group. Table S15. Statistical tests concerning individual CpG sites of H19. (XLSX 1,188 kb)

附加文件1：表S1 临床参数。表S2：133份精子样本中通过直接亚硫酸氢盐测序（DBS）检测得到的MEST、H19、XIST及DDX4基因甲基化水平。表S3：全基因组亚硫酸氢盐测序（WGBS）统计数据。表S4：50个印记调控区域的WGBS甲基化水平。表S5：2764个体细胞-精子差异甲基化区域（DMR）的注释信息及WGBS甲基化水平。表S6：Luján等人2019年报道的差异甲基化区域的WGBS甲基化水平。表S7：NC-NMO差异甲基化区域的注释信息及WGBS甲基化水平。表S8：用于靶向亚硫酸氢盐测序扩增子制备的引物序列。表S9：针对17个NC-NMO差异甲基化区域中通过DBS分析的CpG位点子集（最低覆盖度为5）的平均WGBS甲基化水平。表S10：WGBS与WGBS CpG子集数据（WGBS_s5）的统计学比较。表S11：17个NC-NMO差异甲基化区域的平均DBS甲基化水平。表S12：针对NC-NMO差异甲基化区域的统计学检验结果。表S13：H19基因座中14个CpG位点的甲基化水平。表S14：TG组中T等位基因与G等位基因的CpG3位点甲基化水平。表S15：针对H19基因座单个CpG位点的统计学检验结果。（XLSX格式，文件大小1188 KB）