Immunological indices of Giant African Land snails (Archachatina marginata) improved with fixed dose of vitamin C during acute heat stress

ABSTRACT. The study determined immunological indices of Giant African Land snail (Archachatina marginata) improved with fixed dose of vitamin C under acute heat stress (AHS). Prior to the AHS, vitamin C was administered for four weeks to two treatment groups, while other two treatment groups were not. Each treatment was monitored, haemolymph collected at 0, 30 and 60 minutes exposure times. Immunological cytokines: interferon gamma (IFN-γ) and interleukin 2 (IL-2); and total haemocyte counts (THC) were determined. Under AHS, vitamin C elevated (p 0.05) by vitamin C under AHS. Highest (p 0.05). With fixed dose of vitamin C and exposure time, highest (p摘要：本研究明确了急性热应激（acute heat stress, AHS）条件下，经固定剂量维生素C干预的巨非洲陆生蜗牛（Archachatina marginata）的免疫学指标变化。在施加急性热应激前，两个处理组提前4周摄入固定剂量维生素C，其余两个处理组未进行该预处理。对各处理组开展监测，并在暴露时长分别为0、30、60分钟时采集血淋巴（haemolymph）样本。检测的免疫学指标包括免疫细胞因子（immunological cytokines）：干扰素γ（interferon gamma, IFN-γ）与白细胞介素2（interleukin 2, IL-2），以及总血细胞计数（total haemocyte counts, THC）。在急性热应激条件下，维生素C可提升相关指标（p 0.05）。在急性热应激环境中，维生素C干预组的指标提升效果最为显著（p 0.05）。当采用固定剂量维生素C与暴露时长组合时，最高（p 0.05