Force Profile Analysis Supplementary Files

This folder contains the raw data that was used for quantifications (and the quantifications in an Excel sheet). The raw data (.txt files called EasyQuant files) was extracted from autoradiographs of SDS-PAGE gels using the ImageGauge software from Fuji (associated with the Fuji gel scanner). This machine has since been discontinued (these data were collected 2014-2016). The .txt files were imported into EasyQuant and fitted to a Gaussian distribution automatically by the software (software developed in the Gunnar von Heijne lab by Dr. Rickard Hedman), and the Ffl (fraction full length) was calculated.本文件夹包含用于定量分析的原始数据，相关定量结果已整理至Excel工作表中。 该批次原始数据为格式为.txt的EasyQuant文件，通过富士（Fuji）旗下的ImageGauge软件（配套富士凝胶扫描仪使用），从十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）凝胶的放射自显影图中提取得到。该设备现已停产，相关数据采集于2014至2016年间。上述.txt格式文件被导入EasyQuant软件，由该软件自动拟合为高斯分布（此软件由里克哈德·赫德曼博士于贡纳尔·冯·海因内实验室开发），并计算得到Ffl（全长占比，Fraction Full Length）。