Trypanosoma brucei bloodstream form tagging: Targeted subcellular protein localisation.

Trypanosoma brucei bloodstream form tagging protein localisation data. Widefield epifluorescence microscope images of protein subcellular localisation in the bloodstream form life cycle stage of the unicellular eukaryotic pathogen Trypanosoma brucei by endogenous tagging with mNeonGreen (mNG). This master deposition includes a summary of the localisations, primer sequences and DOI indexing, provided in a directory structure analogous to the TrypTag genome-wide procyclic form project: https://doi.org/10.5281/zenodo.6862298 It does not include any microscopy data, which are spread over multiple Zenodo DOIs. Instead, this deposition and the raw and processed data directories include an index to each DOI. localisations.tsv Tab-delimited table, which can be opened in Excel, of localisation annotations for each gene tagged. Also includes primer sequences used, the 96 well plate in which tagging was carried out organised with one row per gene ID, with sets of columns for N and C terminal tagging. geneselection.tsv Tab-delimited table of criteria used for gene selection for tagging. This includes presence/absence of a Leishmania major or Trypanosoma cruzi ortholog, localisation and signal intensity by procyclic form tagging (TrypTag) and upregulation at mRNA level. id_doi_index.tsv Tab-delimited table listing all Trypanosoma brucei Lister 427 gene IDs, if tagging was attempted at the N or C terminus and, if so, the Zenodo DOI at which to find the microscopy data. To download data for a particular gene ID, find its entry in this table, go to the corresponding Zenodo DOI and download <plateid_date>.zip for the raw microscopy data or <plateid_date>_processed.zip for the processed microscopy data. In the latter, images are named by gene ID and tagged terminus. plate_doi_index.tsv Tab-delimited table listing all 96 plates which were part of the targeted bloodstream form tagging project and the Zenodo DOI at which the data can be found. Downloading the data from all of these Zenodo DOIs gives the full microscopy dataset. trypTag_BSF_master.zip Zip file containing the master directory structure for the targeted bloodstream form tagging project database. This contains all internal code which was used to build the bloodstream form tagging database from the raw microscopy data. readme.docx Documentation on data access and rebuilding the database using trypTag_BSF_master.zip布氏锥虫血流期靶蛋白定位数据集 本数据集包含通过mNeonGreen（mNG）内源标记法，对单细胞真核病原体布氏锥虫（Trypanosoma brucei）血流期生命周期阶段的蛋白亚细胞定位进行成像得到的宽场落射荧光显微镜图像。本核心提交包涵盖定位结果汇总、引物序列及数字对象标识符（DOI）索引，其目录结构与TrypTag全基因组前循环期项目（https://doi.org/10.5281/zenodo.6862298）保持一致。本数据集未包含原始显微镜图像数据，相关图像已分散至多个Zenodo平台的DOI下。本提交包及原始、处理后数据目录仅提供各对应DOI的索引链接。 localisations.tsv：可通过Excel打开的制表符分隔表格，收录所有已标记基因的定位注释信息，同时包含所用引物序列、标记实验所用的96孔板信息（每一行对应一个基因ID，设有N端、C端标记的对应列组）。 geneselection.tsv：制表符分隔表格，记录靶基因筛选的各项标准，包括是否存在利什曼原虫（Leishmania major）或克氏锥虫（Trypanosoma cruzi）的同源基因、基于前循环期标记（TrypTag）得到的定位信息与信号强度，以及mRNA水平的上调情况。 id_doi_index.tsv：制表符分隔表格，列出所有布氏锥虫Lister 427菌株的基因ID，以及是否对其开展了N端或C端标记尝试；若已尝试，则提供对应显微镜图像数据所在的Zenodo DOI。如需下载特定基因ID的相关数据，可在本表中检索对应条目，跳转至相应Zenodo DOI后，下载<板ID_日期>.zip获取原始显微镜图像数据，或下载<板ID_日期>_processed.zip获取处理后的显微镜图像数据。处理后的图像以基因ID与标记末端命名。 plate_doi_index.tsv：制表符分隔表格，列出本靶向血流期标记项目涉及的全部96孔板，以及对应数据所在的Zenodo DOI。下载所有这些Zenodo DOIs中的数据即可获得完整的显微镜图像数据集。 trypTag_BSF_master.zip：包含本靶向血流期标记项目数据库主目录结构的压缩包，内含所有用于从原始显微镜图像数据构建血流期标记数据库的内部代码。 readme.docx：关于数据访问及使用trypTag_BSF_master.zip重建数据库的说明文档。