Data for: Neuroendocrine gene expression coupling of interoceptive bacterial food cues to foraging behavior of C. elegans

Animal internal state is modulated by nutrient intake, resulting in behavioral responses to changing food conditions. The neural mechanisms by which internal states are generated and maintained are not well understood. Here, we show that in the nematode Caenorhabditis elegans, distinct cues from bacterial food – interoceptive signals from the ingestion of bacteria and gustatory molecules sensed from nearby bacteria – act antagonistically on the expression of the neuroendocrine TGF-beta ligand DAF-7 from the ASJ pair of sensory neurons to modulate foraging behavior. A positive-feedback loop dependent on the expression of daf-7 from the ASJ neurons acts to promote transitions between roaming and dwelling foraging states and influence the persistence of roaming states. SCD-2, the C. elegans ortholog of mammalian Anaplastic Lymphoma Kinase (ALK), which has been implicated in the central control of metabolism of mammals, functions in the AIA interneurons to regulate foraging behavior and cel..., , , # Data for: Neuroendocrine gene expression coupling of interoceptive bacterial food cues to foraging behavior of C. elegans

All data is from fluorescence microscopy, C. elegans activity assays, or roaming and dwelling assays.

## Description of the data and file structure

Data is uploaded as .xlsx files and labeled by the figure number in the eLife manuscript.

* Figures 1D, 1E, 1F, 1G, 1H, 3B, 3D, 4A, 4B, 4D, 5A, and 5C represent quantified pdaf-7::gfp expression in ASJ. Data consists of lists of individual maximum GFP fluorescence values in ASJ normalized to expression time for individual animals. Quantification was performed in FIJI and is in arbitrary brightness units.
* Figures 2A right, 2C right, 4E right, 4G right, and 4J right are data from exploration assays. The exploration assay was performed loosely as previously described with several modifications (Flavell et al., 2013). Thirty-five millimeter NGM plates with no peptone were seeded with 500 µL OP50 grown overnight ...

动物的内感受状态受营养摄入调控，进而对食物环境的变化产生行为响应。目前，内感受状态的产生与维持的神经机制尚未完全阐明。本研究显示，在秀丽隐杆线虫（Caenorhabditis elegans）中，来自细菌食物的两类不同信号——摄取细菌产生的内感受信号，以及感知邻近细菌的味觉分子——对ASJ感觉神经元对表达的神经内分泌转化生长因子β（Transforming Growth Factor beta, TGF-β）配体DAF-7产生拮抗调控，以此调节觅食行为。依赖ASJ神经元DAF-7表达的正反馈环路可促进漫游与停留两种觅食状态间的转换，并影响漫游状态的持续时长。SCD-2是哺乳动物间变性淋巴瘤激酶（Anaplastic Lymphoma Kinase, ALK）的秀丽隐杆线虫同源蛋白，该激酶已被证实参与哺乳动物代谢的中枢调控，其在AIA中间神经元中发挥功能以调控觅食行为，且……，，，# 数据集主题：耦合内感受性细菌食物信号与秀丽隐杆线虫觅食行为的神经内分泌基因表达

所有实验数据均来自荧光显微镜成像、秀丽隐杆线虫活性检测以及漫游/停留觅食状态检测。

## 数据集与文件结构说明

数据集以.xlsx格式上传，并按eLife投稿论文中的图号进行命名标注。
* 图1D、1E、1F、1G、1H、3B、3D、4A、4B、4D、5A及5C的数据为ASJ神经元中pdaf-7::gfp的定量表达结果。该数据集包含单个动物ASJ神经元的GFP（Green Fluorescent Protein）最大荧光强度值列表，数值已按个体动物的表达时长进行归一化处理。定量分析在FIJI软件中完成，结果以任意亮度单位表示。
* 图2A右侧、2C右侧、4E右侧、4G右侧及4J右侧的数据来自探索行为检测实验。该探索实验参照已发表方案并做了若干修改（Flavell等，2013）完成：使用不含蛋白胨的35mm NGM培养基平板，接种500μL过夜培养的OP50菌液……