Analysis of the multiplex real-time PCR assay.

Multiplex qPCR assays were performed containing both primer and probe sets for all four targets or primer and probe set for single target. Analysis 1 shows data from multiplex assay analyzed as multiplex where all four targets were analyzed simultaneously. Analysis 2 shows data from multiplex assay but data was analyzed as a single assay for each target. Analysis 3 shows data from single assays.本研究开展了多重定量聚合酶链反应（multiplex qPCR）实验，实验所用引物与探针组合分为两类：一类针对全部四个靶标，另一类仅针对单个靶标。分析1展示的数据来自多重qPCR实验，且采用多重分析模式，即同步对全部四个靶标进行检测分析。分析2所用数据同样来源于多重qPCR实验，但分析过程中将其按单个靶标的单重实验分别进行处理。分析3则展示了单重qPCR实验的检测数据。