Resistance of DEPN-8+1.5% by weight Mini-B to degradation by phospholipase A2 (PLA2) compared to calf lung surfactant extract (CLSE).

Data are mean±SEM for n = 3. DEPN-8+1.5% by weight Mini-B was incubated in vitro with PLA2 (0.1 Units/ml) for 30 min at 37°C, and degradation was assessed by measuring lipid classes in weight percent based on phosphate analysis of bands on thin layer chromatography. Results for CLSE in the presence and absence of PLA2 utilized identical methods as reported previously by Wang et al [30].实验数据以均值±标准误（mean±SEM）表示，样本量n=3。将按重量计含1.5% DEPN-8的Mini-B制剂与磷脂酶A2（PLA2，0.1单位/毫升）在37℃条件下体外孵育30分钟，通过基于薄层色谱（thin layer chromatography）条带的磷酸分析方法测定各脂质类别的重量百分比，以此评估其降解程度。针对存在与不存在PLA2时的CLSE相关实验结果，其采用的实验方法与Wang等人此前在文献[30]中报道的方法完全一致。