Original paired-end transcriptome outputs from the RNAseq analyses of the deep-sea zoanthid Zibrowius primnoidus

This dataset contains outputs generated from the original paired-end transcriptomic analyses of the deep-sea zoanthid Zibrowius primnoidus. The dataset includes the following files:

• 155835_ID3533_1-1_S111_L004_R1_001.fastq.gz.P.qtrim.gz: Preprocessed forward reads used for the de novo assembly, obtained from paired-end RNA-Seq data of Zibrowius primnoidus, containing high-quality sequences that passed quality trimming and filtering with Trimmomatic.

• 155835_ID3533_1-1_S111_L004_R2_001.fastq.gz.P.qtrim.gz: Preprocessed reverse reads used for the de novo assembly, obtained from paired-end RNA-Seq data of Zibrowius primnoidus, containing high-quality sequences that passed quality trimming and filtering with Trimmomatic.

• Zibrowius_primnoidus_RF_assembly.Trinity.fasta: Original, non-filtered de novo paired-end transcriptome assembly of Zibrowius primnoidus, generated from ~90 million PE reads using the Trinity assembler.

• Zibrowius_primnoidus_RF_assembly.Trinity.fasta_stats.txt: Statistical summary of the paired-end transcriptome assembly of Zibrowius primnoidus (e.g. number of transcripts, N50, GC content).

• Zibrowius_primnoidus_RF_assembly.Trinity.fasta.gene_trans_map: Transcript-to-gene mapping file generated during the Trinity paired-end transcriptome assembly of Zibrowius primnoidus.

• quant_Zp.sf: Salmon output file containing expression estimates (transcript-level abundance) for the assembled transcripts from the paired-end RNA-Seq data of Zibrowius primnoidus.

Additionally, the dataset includes the following files from DIAMOND BLASTx analyses, which used the original de novo paired-end transcriptome assembly of Zibrowius primnoidus:

• Zibrowius_primnoidus_assembly.Trinity.blastx.outfmt6: BLASTx output file against the UniRef90 database, reporting the top alignment for each query (assembled transcript) from the paired-end assembly of Zibrowius primnoidus.

• Zibrowius_primnoidus_assembly.Trinity.blastx.outfmt6.grouped: Grouped BLASTx hits from the paired-end assembly of Zibrowius primnoidus, designed to improve sequence coverage by combining multiple high-scoring segment pairs (HSPs).

• Zibrowius_primnoidus_assembly.Trinity.blastx.outfmt6.hist: Histogram summarizing the distribution of BLASTx hit lengths obtained from the paired-end assembly of Zibrowius primnoidus.

• Zibrowius_primnoidus_assembly.Trinity.blastx.outfmt6.w_pct_hit_length: File providing percentages of hit lengths from BLASTx analyses of the paired-end assembly of Zibrowius primnoidus, including each top hit’s length and the percentage of that length covered in the alignment.

本数据集包含深海群体海葵Zibrowius primnoidus的原始双端转录组分析生成结果。数据集包含以下文件：
• 155835_ID3533_1-1_S111_L004_R1_001.fastq.gz.P.qtrim.gz：用于从头组装的预处理正向reads，源自Zibrowius primnoidus的双端RNA测序数据，包含经Trimmomatic完成质量修剪与过滤后的高质量序列。
• 155835_ID3533_1-1_S111_L004_R2_001.fastq.gz.P.qtrim.gz：用于从头组装的预处理反向reads，源自Zibrowius primnoidus的双端RNA测序数据，包含经Trimmomatic完成质量修剪与过滤后的高质量序列。
• Zibrowius_primnoidus_RF_assembly.Trinity.fasta：Zibrowius primnoidus的原始非过滤双端转录组从头组装结果，使用Trinity组装软件从约9000万条双端reads生成。
• Zibrowius_primnoidus_RF_assembly.Trinity.fasta_stats.txt：Zibrowius primnoidus双端转录组组装的统计汇总文件（例如转录本数量、N50值、GC含量）。
• Zibrowius_primnoidus_RF_assembly.Trinity.fasta.gene_trans_map：Zibrowius primnoidus双端转录组Trinity组装过程中生成的转录本-基因映射文件。
• quant_Zp.sf：Salmon输出文件，包含Zibrowius primnoidus双端RNA测序数据组装转录本的转录本水平丰度估计值。

此外，数据集还包含基于DIAMOND BLASTx分析生成的以下文件，该分析以Zibrowius primnoidus的原始双端转录组从头组装结果为参考序列：
• Zibrowius_primnoidus_assembly.Trinity.blastx.outfmt6：针对UniRef90数据库的BLASTx输出文件，报告Zibrowius primnoidus双端组装转录本（查询序列）的最优比对结果。
• Zibrowius_primnoidus_assembly.Trinity.blastx.outfmt6.grouped：Zibrowius primnoidus双端组装序列的分组BLASTx比对结果，通过合并多个高分片段对（High-scoring Segment Pair，HSPs）以提升序列覆盖度。
• Zibrowius_primnoidus_assembly.Trinity.blastx.outfmt6.hist：汇总Zibrowius primnoidus双端组装序列BLASTx比对片段长度分布的直方图文件。
• Zibrowius_primnoidus_assembly.Trinity.blastx.outfmt6.w_pct_hit_length：提供Zibrowius primnoidus双端组装序列BLASTx分析比对长度占比的文件，包含每条最优比对的比对长度及该长度在整体比对中的覆盖百分比。