P301S Mutant Tau-Driven Zebrafish Platform Enables Systematic Identification of Pharmacological GSK3ß Inhibitors with Anti-Neurodegenerative Efficacy

Spatial transcriptome sequencing technology has provided high-resolution transcriptome data for studying the molecular mechanism of Tau P301S mediating Alzheimer's disease-like pathological changes in zebrafish, providing a unique insight into the pathogenesis of tau pathology-mediated cell-cell interactions in AD. Overall design: This study utilized four Danio rerio lines: wild-type (WT) and three transgenic lines (AD3, AD4, AD5) expressing human Tau protein with the P301S mutation (Tau-P301S). All transgenic lines were generated via stable integration of the human TAU P301S transgene.