Processed Vessel Quantification Data for 28-day Bijel-Templated Implant Study in Nude Mice

Immunohistochemistry was performed on subcutaneous tissue sections around Bijel-Templated Material (BTM) and Particle-Templated Material (PTM) implants after 28 days in nude mice. Blood vessels were labeled with the endothelial cell marker CD31 and the pericyte marker alphaSMA. Tissue sections were imaged via fluorescence microscopy and vessel outlines residing within implants were traced and quantified. This dataset contains the area of traced vessels within BTM and PTM implants sorted in 200 micrometer bins relative to the implant boundary. Additionally, this dataset contains the total vessel area in per mouse per total tissue area in the corresponding 200 micrometer bin for BTM and PTM implants. Individual and average total vessel area (n=4, mean +/- standard error of the mean) were plotted versus 200 micrometer bins from implant boundary.

本数据集对应的实验流程如下：将毕耶尔模板材料（Bijel-Templated Material, BTM）与颗粒模板材料（Particle-Templated Material, PTM）植入裸鼠（nude mice）体内28天后，对两类植入物周围的皮下组织切片（subcutaneous tissue sections）开展免疫组织化学（Immunohistochemistry）染色。使用内皮细胞标志物CD31与周细胞标志物α-SMA（alphaSMA）对血管进行标记，通过荧光显微镜（fluorescence microscopy）对组织切片进行成像，随后描记并定量植入物内部的血管轮廓。本数据集包含按距植入物边界的200微米区间分组排序的BTM与PTM植入物内描记血管的面积数据；此外，数据集还收录了针对BTM与PTM植入物，每只小鼠在对应200微米区间内的总血管面积与该区间总组织面积的比值数据。将单个血管面积值及总血管面积平均值（n=4，平均值±标准误）绘制为与距植入物边界200微米区间的关系图。