DIFFERENT PROTOCOLS OF POSTCONDITIONING DOES NOT ATTENUATE MESENTERIC ISCHEMIA-REPERFUSION INJURY AFTER SHORT-TERM REPERFUSION

ABSTRACT Background: Mesenteric ischemia is a challenging diagnosis. Delay in diagnosis can lead to extent bowel necrosis and poor outcomes. Ischemia and reperfusion syndrome plays an important role in this scenario. Aim: To access effects of different post-conditioning cycles on mesenteric ischemia-reperfusion syndrome. Method: Twenty-five rats were assigned into five groups: Sham, used to establish normal parameters; control group, submitted to mesenteric ischemia for 30 min; in groups GP3, GP1 and GP30, ischemia was followed by post-conditioning protocol, which consisted of 1 cycle of 3 min (GP3), 3 cycles of 1 min (GP1) or 6 cycles of 30 s (GP30), respectively. Ileum samples were harvested after one hour of reperfusion. Intestinal mucosal injury was evaluated through histopathological analysis. Results: The average of mesenteric injury degree was 0 in the sham group, 3.6 in the control group, 3.4 in GP3, 3.2 in GP1, and 3.0 in GP30; villous length average was 161.59 in sham group, 136.27 in control group, 135.89 in GP3, 129.46 in GP1, and 135.18 in GP30. Was found significant difference between sham and other groups (p摘要 背景：肠系膜缺血（mesenteric ischemia）是一类极具挑战性的临床诊断难题。诊断延误可引发大范围肠坏死并导致不良预后，而缺血再灌注综合征（ischemia-reperfusion syndrome）在此病理过程中扮演着重要角色。研究目的：评估不同后处理周期对肠系膜缺血再灌注综合征的干预效果。研究方法：将25只大鼠随机分为5组：假手术（Sham）组，用于建立正常生理参考参数；对照组，接受30分钟肠系膜缺血造模；GP3、GP1及GP30组，均在缺血后实施后处理方案，具体分别为1次3分钟循环、3次1分钟循环以及6次30秒循环。再灌注1小时后采集回肠样本，通过组织病理学分析（histopathological analysis）评估肠黏膜损伤程度。研究结果：假手术组肠系膜损伤程度平均值为0，对照组为3.6，GP3组为3.4，GP1组为3.2，GP30组为3.0；肠绒毛长度平均值方面，假手术组为161.59，对照组为136.27，GP3组为135.89，GP1组为129.46，GP30组为135.18。假手术组与其余各组间存在显著统计学差异（p