High occurrence of jellyfish predation by black-browed and Campbell albatross identified by DNA metabarcoding

This spreadsheet provides the sequences counts for the DNA groups found in the scats of black-browed albatross at New Island and Steeple Jason Island, Falkland Islands; Diego Ramírez and Albatross Islet, Chile; Bird Island, South Georgia; Canyon des Sourcils Noirs, Kerguelen Archipelago, France; Macquarie Island, Australia; and Campbell albatross at Campbell Island, NZ.Scat samples were collected in 2013/14 and 2014/15 at New Island, Steeple Jason Island, Macquarie Island, Campbell Island and Bird Island; in 2013/14 and 2015/16 at Kerguelen; in 2014/15 and 2015/16 at Albatross Islet and in 2013/14 at Diego Ramírez. Samples were collected during Incubation (Oct-Nov), early chick-rearing (Dec-Jan) or late-chick rearing (Feb-Mar). Due to the availability of birds at the colony, samples were predominantly collected from adults during incubation and early chick-rearing and chicks during late chick rearing. Samples sizes were too low during this study to directly compare dietary differences between chicks and adults; however, dietary comparisons between breeding stages were examined for sites where samples were collected during multiple breeding stages.Samples were PCR amplified with a universal metazoan primer set that is highly conserved and amplifies a region of the nuclear small subunit ribosomal DNA gene (18S rDNA). Details of the molecular methods and synthesis of this data can be found in: McInnes, J.C., Alderman, R., Raymond, B., Lea, M-A., Deagle, B., Catry, P., Gras, M., Phillip, R.A., Stanworth, A., Suazo, C., Thompson, D., Weimerskirch, H., Gras. M., and Jarman, S.N. High occurrence of jellyfish predation by black-browed and Campbell albatross identified by DNA metabarcoding. Molecular Ecology.

本电子表格提供了在福克兰群岛新岛与陡杰森岛、智利迭戈拉米雷斯岛与信天翁岛、南乔治亚伯德岛、法属凯尔盖朗群岛黑泉峡谷（Canyon des Sourcils Noirs）、澳大利亚麦夸里岛采集的黑眉信天翁，以及新西兰坎贝尔岛采集的坎贝尔信天翁粪样中检出的DNA类群序列计数数据。

粪样采集信息如下：2013/14年度与2014/15年度于新岛、陡杰森岛、麦夸里岛、坎贝尔岛及伯德岛；2013/14年度与2015/16年度于凯尔盖朗群岛；2014/15年度与2015/16年度于信天翁岛；2013/14年度于迭戈拉米雷斯岛。

样本采集时段涵盖繁殖期（10-11月）、雏鸟早期抚育期（12-1月）与雏鸟晚期抚育期（2-3月）。受繁殖群中鸟类可及性限制，样本主要采集自繁殖期与雏鸟早期抚育期的成鸟，以及雏鸟晚期抚育期的雏鸟。

本研究样本量有限，无法直接比较雏鸟与成鸟的饮食差异；但针对多个繁殖阶段均采集到样本的研究位点，可开展不同繁殖阶段间的饮食对比分析。

所有样本均采用保守性极强的通用后生动物引物组进行PCR扩增，可靶向扩增核小亚基核糖体DNA（18S rDNA）的特定区域。

本研究的分子实验方法与数据整合细节可参阅以下文献：McInnes JC, Alderman R, Raymond B, Lea M-A, Deagle B, Catry P, Gras M, Phillip RA, Stanworth A, Suazo C, Thompson D, Weimerskirch H, Gras M, Jarman SN. 基于DNA宏条形码（DNA metabarcoding）技术鉴定黑眉信天翁与坎贝尔信天翁捕食水母的高发生率. 《分子生态学》（Molecular Ecology）.