Real-time quantitative PCR analysis of PP2C genes in Brachypodium distachyon of different tissues and under multiple stresses

PP2C genes responding to 13 stresses were screened using qPCR. All sampes were repeated with 3 times and ACTIN were regarded as house keeping gene.The stresses including heavy metal treatments, environmental factors,phytohormones and biotic treatments. qPCR gene expression profiling. The 2 weeks-seedlings were used and 2 µg of total RNA were used for cDNA synthesis by using PrimeScript Reverse Transcriptase (TAKARA).