Dietary intake regulates the circulating pro-inflammatory monocyte pool

Caloric restriction is considered to be anti-inflammatory. In this study we examined the effect of fasting on peripheral leukocyte populations. We found that short-term fasting affects metabolic and pro-inflammatory activity of monocytes and decreases numbers of circulating monocytes. For this study, we sorted hepatocytes from fed and fasted mice, monocytes from bone marrow from fed and fasted mice, and monocytes from bone marrow from wildtype and Ccr2-deficient mice. 39 samples; 2-4 replicates per condition