Organelle-target discovery for selective amoebicidal therapy: three-endpoint screen vs Naegleria fowleri (data & code) + Montenegro's Medium (MM) protocol, v1.0

Complete package for the organelle-target screen against Naegleria fowleri. Includes S1–S24 Tables.xlsx (plate maps/readouts), minimal Python scripts to rebuild Figs 1–3 (commands in README), and Montenegro’s Medium (MM) protocol used in all assays (1 L formula in g/L, target pH 6.8 ± 0.2, 0.22 µm filtration only, storage/QC notes). Endpoints: LDH at 24 h, caspase-3 at 48 h, JC-1 at 72 h for amoeba-only and HeLa + N. fowleri plates. Per-plate normalization used NoRx = 0% and Positive = 100% with inversion when (Positive − NoRx) < 0; when anchors overlapped, raw signals are provided and anchors logged. Cite this version DOI in manuscripts; link the paper later under Related works → “IsSupplementTo”. Version: v1.0.针对福氏耐格里阿米巴（Naegleria fowleri）的细胞器靶向筛选完整数据集包。内含S1至S24号表格Excel文件（板位图与读数文件）、用于复现图1至图3的极简Python脚本，其运行命令详见README文档；同时包含所有实验所用的黑山培养基（Montenegro’s Medium，MM）实验方案，具体涵盖1升装配方的质量浓度（单位：g/L）、目标pH值6.8±0.2、仅可使用0.22μm滤膜过滤，以及储存与质量控制说明。针对仅阿米巴培养板与HeLa细胞+福氏耐格里阿米巴共培养板，实验终点检测指标及对应时间为：24小时检测乳酸脱氢酶（LDH）、48小时检测半胱氨酸天冬氨酸蛋白酶3（caspase-3）、72小时检测JC-1荧光信号。每块培养板采用归一化处理：以无处理组（NoRx）为0%基准、阳性对照组（Positive）为100%基准，当（阳性组信号值 - 无处理组信号值）< 0时，需对信号进行反转；若两组锚定基准出现信号重叠，则提供原始信号值并记录锚定相关信息。投稿时请引用本数据集版本的DOI；后续可在相关成果（Related works）板块下通过"IsSupplementTo"关联该研究论文。版本：v1.0。