Identification of polysomal-association changes upon ablation of cytoplasmic SRSF1

SRSF1, shuttles between the nucleus and cytoplasm affecting post-splicing processes. However, the physiological significance of this remains unclear. Here, we used genome editing to knock-in a nuclear retention signal (NRS) in Srsf1 to create a mouse model harboring a non-shuttling SRSF1 protein. We then assessed how alblation of shuttling activities of SRSF1 affects mRNA translation. Overall design: Sequencing of total, monosomal, polysomal RNA from ESC, MEF and NSC cells expressing wild type or nuclear retained SRSF1