Quantification of yurJ and msmX mRNA level in the B. subtilis wild-type strain.

The results represent the fold-change of the expression in the target conditions (T) versus the control conditions (C). (glu), (ara) and (A3) indicate the presence of 0.1% of glucose, arabinose or arabinotriose, respectively. Primers ARA638 and ARA639 were used for yurJ and primers ARA640 and ARA641 for msmX. Fold-change was normalized using primers ARA583 and ARA584 for the 16S gene. Statistical analyses were performed with GraphPad Prism (version 5.00) using Ct values obtained from three independent assays. p values were determined using an unpaired t test (ns, non-significant difference; *, p p p本研究结果展示了目标条件（T）相较于对照条件（C）下的基因表达倍数变化（fold-change）。其中(glu)、(ara)与(A3)分别代表体系中含有0.1%的葡萄糖（glucose）、阿拉伯糖（arabinose）或阿拉伯三糖（arabinotriose）。针对yurJ基因，实验采用引物ARA638与ARA639进行扩增；针对msmX基因，则采用引物ARA640与ARA641。基因表达倍数变化以靶向16S基因的引物ARA583与ARA584作为内参进行标准化处理。统计分析借助GraphPad Prism（5.00版本）完成，所用数据为三次独立实验获取的Ct（循环阈值）值。p值通过非配对t检验计算得到（ns：无显著性差异；*，p<0.05；**，p<0.01；***，p<0.001）。