CIL:41367

The image shows a portion of a slice through a 3D tomographic reconstruction of a mouse adenocarcinoma cell based on x-ray microscopy. Live cells were cryofixed by plunge freezing in liquid ethane and the vitrified material observed unstained using x-ray microscopy at a wavelength that yields high contrast between water and organic material. Images were taken over a range of specimen tilts from -60 to +60 degrees at 1 degree intervals. The image represents a 9.8 nm thick slice of the computed tomogram in the x-y plane, and shows the plasma membrane with cytoplasm above and medium below. Numerous vesicles are present in the cytoplasm. The image has a resolution of ~36 nm (Rayleigh Criterion) and ~70 nm (Fourier Ring Correlation), the latter corresponding to ~35 nm half-pitch resolution. Additional X-ray images from the study are included in this CIL Group. See also: W G Muller et al. 2012 Towards an atlas of mammalian cell ultrastructure by soft X-ray tomography. J Struct Biol 177:179-192.

本图像为基于X射线显微镜（X-ray microscopy）构建的小鼠腺癌细胞三维断层重建切片的局部视图。活细胞通过浸入液态乙烷的快速冷冻法完成冷冻固定，未经过染色处理的玻璃态样品使用对水与有机物间可产生高对比度的X射线进行观测。图像采集时，样品倾斜角度范围为-60°至+60°，以1°为间隔采集图像。本图像对应计算机断层重建图像在x-y平面上厚度为9.8 nm的切片，可见细胞膜（plasma membrane），其上方为细胞质（cytoplasm），下方为细胞培养基。细胞质内可见大量囊泡（vesicles）。本图像分辨率约为36 nm（基于瑞利判据（Rayleigh Criterion））与70 nm（基于傅里叶环相关法（Fourier Ring Correlation）），后者对应约35 nm的半节距分辨率。本CIL群组（CIL Group）还收录了该研究的其他X射线图像。另可参阅：W G Müller 等（2012）《借助软X射线断层成像构建哺乳动物细胞超微结构图谱》，《结构生物学杂志》（J Struct Biol），177卷：179-192。