Quantification of the N-glycosylation site occupancy.

The N-glycosylation site occupancy was calculated from the comparison of the ion intensity of the non-glycosylated versus deglycosylated peptides using single and discharged specific ions and from densitometry analysis of the two bands corresponding to heavy chains observed on the NuPAGE gel.Quantification of the N-glycosylation site occupancy.N-糖基化位点占有率（N-glycosylation site occupancy）可通过两种途径计算：其一，借助单一特异性离子与解离特异性离子，对比非糖基化肽段与去糖基化肽段的离子强度；其二，对NuPAGE凝胶上观测到的对应重链的两条条带开展光密度分析。N-糖基化位点占有率的定量分析。