Gene expression profiling following the overexpression or the knockdown of Ambra1 in A375 melanoma cell lines

Melanoma is the most deadly type of skin cancers and, there is a continuous need to develop early biomarkers as well as treatments to improve the survivability of patients. Ambra1 is an essential regulator in autophagy and it has been recently shown to have a role in cell proliferation. We used microarrays to analyse the transcriptomic changesi n A375 melanoma cell lines following Ambra1 differential expression Four stably transfected A375 melanoma cell lines were utilized for this study. Overexpressions of Ambra1 and its matching control, β-galactosidase were performed by retroviral infection. While knockdown of Ambra1 was performed by lentiviral infection that expresses a shRNA construct targeted to Ambra1 and the control cell line expressed a scrambled shRNA control. Total RNA was extracted and hybridized on Affymetrix microarrays. Three biological replicates of each cell line were used for eah group.