The combined effects of multiple invasive species on persistence of imperiled Pahrump Poolfish

Many ecosystems have been invaded by more than one non-native species, but research evaluating the combined effects of multiple invasive species has been limited. In the southwest USA, many aquatic systems have been invaded by multiple species such as non-native crayfish and non-native fishes. We used experimental mesocosms to test individual and combined effects of invasive Red Swamp Crayfish, Procambarus clarkii, and Western Mosquitofish, Gambusia affinis, on endangered Pahrump Poolfish, Empetrichthys latos. We found that crayfish alone reduced adult poolfish survival; however, crayfish by themselves had moderate but non-significant impacts on the production of poolfish juveniles. Mosquitofish had no effect on the survival of poolfish adults, but significantly reduced recruitment of juveniles. When both crayfish and mosquitofish were present, both adult survival and juvenile production were significantly decreased. These findings were consistent with the recent decline of a wild poolf..., Western Mosquitofish were obtained from Sutter-Yuba Mosquito and Vector Control district in Yuba City, CA. Poolfish used in this experiment included a mixture of wild poolfish and lab-reared poolfish. The wild poolfish were collected from on 13 June 2017 from Shoshone Stock Pond (White Pine County, NV) while the lab-reared poolfish were descendants from poolfish originally collected in 2014 from Spring Mountain Ranch State Park, Clark County [31]. Red Swamp Crayfish were sourced from Carolina Biological suppliers Burlington, NC.
 Three fish communities were used in this experiment forming a single block within a randomized block design. Each of seven blocks contained a total of three mesocosms including the following treatments: I.) allopatric poolfish, II.) poolfish sympatric with crayfish, and III.) poolfish sympatric with both mosquitofish and crayfish. We did not include a poolfish + mosquitofish treatment because of the limited number of poolfish available for this experiment...., , # Crayfish and mosquitofish impacts on experimental poolfish populations

This readme file was generated on 2024/12/20 by Craig Stockwell

## GENERAL INFORMATION

Title of Dataset:

Author/Principal Investigator Information
Name: Brandon Paulson
ORCID: [https://orcid.org/0000-0002-4635-1485](https://orcid.org/0000-0002-4635-1485)
Institution: North Dakota State University
Address: Biological Sciences, Stevens Hall 201, NDSU Box 6050, Fargo, ND 58108
Email: [[e-mail]](mailto:[e-mail])

Author/Associate or Co-investigator Information
Name: Craig Alan Stockwell
ORCID: [https://orcid.org/0000-0001-7882-8674](https://orcid.org/0000-0001-7882-8674)
Institution: North Dakota State University
Address: Biological Sciences, Stevens Hall 201, NDSU Box 6050, Fargo, ND 58108
Email: [[e-mail]](mailto:[e-mail])

Date of data collection:
Experiment with Poolfish_Crayfish and Mosquitofish: 2017-06-01 to 2018-08-10
Geographic location...,

已有诸多生态系统遭受多种非本土物种入侵，但针对多种入侵物种联合效应的相关研究仍较为匮乏。在美国西南部，众多水生生态系统已被非本土螯虾与非本土鱼类等多个物种入侵。本研究借助中型实验生态系统（experimental mesocosms），探究了入侵物种红沼泽螯虾（Red Swamp Crayfish, *Procambarus clarkii*）与西部食蚊鱼（Western Mosquitofish, *Gambusia affinis*）单独及联合作用对濒危物种帕拉普普鳉（Pahrump Poolfish, *Empetrichthys latos*）的影响。

研究结果显示：单独的红沼泽螯虾可降低成年帕拉普普鳉的存活率；但其对幼鳉的繁育仅存在温和且不显著的影响。西部食蚊鱼对成年帕拉普普鳉的存活率无显著作用，但可显著降低幼鳉的补充量。当红沼泽螯虾与西部食蚊鱼同时存在时，成年鳉的存活率与幼鳉的繁育量均出现显著下降。上述研究结果与野生帕拉普普鳉种群近期的衰退趋势相符……

实验所用西部食蚊鱼采集自加利福尼亚州尤巴城的萨特尔-尤巴蚊虫与媒介防控区（Sutter-Yuba Mosquito and Vector Control district）。本实验中的帕拉普普鳉包含野生个体与实验室繁育个体两类：野生个体于2017年6月13日采自内华达州怀特松县的肖肖尼贮水池（Shoshone Stock Pond）；实验室繁育个体为2014年采自克拉克县斯普林山牧场州立公园的帕拉普普鳉种群的后代[31]。红沼泽螯虾购自北卡罗来纳州伯灵顿的卡罗来纳生物供应商公司（Carolina Biological suppliers）。

本实验采用三类鱼类群落组合，在随机区组设计框架下构成单个实验区组。全部7个区组均各包含3个中型实验生态系统，分别对应以下处理组：I. 异域分布的帕拉普普鳉；II. 与红沼泽螯虾同域共存的帕拉普普鳉；III. 与西部食蚊鱼和红沼泽螯虾均同域共存的帕拉普普鳉。由于本实验可用的帕拉普普鳉数量有限，我们未设置仅包含帕拉普普鳉与西部食蚊鱼的处理组……

# 螯虾与食蚊鱼对实验帕拉普普鳉种群的影响
本说明文件由Craig Stockwell于2024年12月20日生成

## 基本信息
### 数据集标题：
### 项目负责人信息
姓名：Brandon Paulson
ORCID：[https://orcid.org/0000-0002-4635-1485](https://orcid.org/0000-0002-4635-1485)
所属机构：北达科他州立大学（North Dakota State University）
通讯地址：生物科学系，史蒂文斯楼201室，NDSU信箱6050，法戈市，北达科他州58108
电子邮箱：[[e-mail]](mailto:[e-mail])

### 联合研究者信息
姓名：Craig Alan Stockwell
ORCID：[https://orcid.org/0000-0001-7882-8674](https://orcid.org/0000-0001-7882-8674)
所属机构：北达科他州立大学（North Dakota State University）
通讯地址：生物科学系，史蒂文斯楼201室，NDSU信箱6050，法戈市，北达科他州58108
电子邮箱：[[e-mail]](mailto:[e-mail])

### 数据收集日期：
帕拉普普鳉、螯虾与食蚊鱼相关实验：2017-06-01 至 2018-08-10
地理位置：……