Additional file 1 of Chalkiness and premature controlled by energy homeostasis in OsNAC02 Ko-mutant during vegetative endosperm development

Additional file 1: Figure S1. The mutant tags of target sites and mutants detection by PCR sequencing. Figure S2. The yield characters of T2 N2 /N3 mut (95 DAG) in field (95 DAG in 2021, 125 DAG in 2020). Figure S3. The homologous clusters of OsNAC family genes by phylogenetic analysis. Figure S4. The homologous analysis of NAC in plant. The Gene ID are according to the database RAP (rapdb.dna.affrc.go.jp), and (OsNAC02, Os02g0594800), (OsNAC06, Os06g0267500) in NCBI of homologous analysis. Figure S5. The ABRE/ G-box motif locations on the promoter regions of OsNAC02 regulated genes. Figure S6. The chalky phenotype of N2/N3 mut seeds (T2) in 2021. Figure S7. The histological analysis during endosperm development. Figure S8. The PCA clusters based on RNA-seq analysis. Figure S9. The heatmap diagram of DEGs in N2/N3 versus WT. Figure S10. The genes of 39 ECs in Sucrose and Starch metabolic pathway in Mapman of N2 vs WT seeds (3DAP) are verified by qRT-PCR. Figure S11. The antagonism between energy storage and development requirement under stress in Ko-Osnac02 mutant.

附加文件1：图S1。靶位点的突变标签及基于PCR测序的突变体检测。图S2：2021年取样于发芽后95天（Days After Germination, DAG）、2020年取样于发芽后125天（DAG）的T2代N2/N3突变体的田间产量性状。图S3：基于系统发育分析的OsNAC家族基因同源聚类。图S4：植物中NAC基因的同源性分析。本分析所用基因编号依据RAP数据库（rapdb.dna.affrc.go.jp），且同源性分析中的(OsNAC02, Os02g0594800)、(OsNAC06, Os06g0267500)均来自NCBI数据库。图S5：OsNAC02调控基因启动子区域的ABRE/G-box基序定位。图S6：2021年N2/N3突变体T2代种子的垩白表型。图S7：胚乳发育过程的组织学分析。图S8：基于RNA-seq分析的主成分分析（Principal Component Analysis, PCA）聚类结果。图S9：N2/N3与野生型（Wild Type, WT）间差异表达基因（Differentially Expressed Genes, DEGs）的热图。图S10：对N2与野生型种子（开花后3天，Days After Pollination, 3DAP）蔗糖与淀粉代谢通路中经Mapman分析得到的39个表达聚类基因，通过qRT-PCR（quantitative Real-Time PCR, qRT-PCR）进行验证。图S11：Ko-Osnac02突变体中能量储存与胁迫下发育需求间的拮抗作用。