Transcriptome sequencing and screening of genes related to glucose availability in Schizosaccharomyces pombe by RNA-seq analysis

Abstract While calorie restriction is the most used experimental intervention to increase lifespan in numerous model organisms, increasing evidence suggests that excess glucose leads to decreased lifespan in various organisms. To fully understand the molecular basis of the pro-aging effect of glucose, it is still important to discover genetic interactions, gene expression patterns, and molecular responses depending on glucose availability. Here, we compared the gene expression profiles in Schizosaccharomyces pombe mid-log-phase cells grown in three different Synthetic Dextrose media with 3%, 5%, and 8% glucose, using the RNA sequencing method. Expression patterns of genes that function in carbohydrate metabolism were downregulated as expected, and these genes were downregulated in line with the increase in glucose content. Significant and consistent changes in the expression were observed such as genes that encoding retrotransposable elements, heat shock proteins, glutathione S-transferase, cell agglutination protein, and conserved fungal proteins. We group some genes that function together in the transcription process and mitotic regulation, which have recently been associated with glucose availability. Our results shed light on the relationship between excess glucose, diverse cellular processes, and aging.摘要：尽管热量限制是目前在多种模式生物中用于延长寿命的最常用实验干预手段，但越来越多的研究证据表明，高葡萄糖暴露会缩短多种生物的寿命。为了全面阐明葡萄糖促衰老效应的分子机制，探究依赖于葡萄糖可获得性的遗传互作、基因表达模式以及分子应答仍具有重要研究价值。本研究采用RNA测序（RNA sequencing）技术，对比了分别在含3%、5%、8%葡萄糖的三种不同合成葡萄糖培养基（Synthetic Dextrose media）中培养的粟酒裂殖酵母（Schizosaccharomyces pombe）对数中期细胞的基因表达谱。正如预期，参与碳水化合物代谢的基因表达被下调，且其下调程度随葡萄糖浓度升高而递增。研究观察到一批基因的表达发生了显著且一致的变化，包括编码逆转录转座因子、热激蛋白、谷胱甘肽S-转移酶、细胞凝集蛋白以及保守真菌蛋白的基因。我们对近期被发现与葡萄糖可获得性相关的、参与转录过程与有丝分裂调控的协同功能基因进行了聚类分组。本研究结果为解析高葡萄糖、多种细胞进程与衰老之间的内在关联提供了新的理论依据。