Endothelial progenitor cells contribute to neovascularization of non-small cell lung cancer via HDAC7 mediated cytoskeleton regulation and angiogenic genes transcription

To supply tumor tissues with nutrients and oxygen, endothelial progenitor cells (EPCs) are recruited to tumor bed and contribute to tumor neovascularization. HDAC7 is a critical regulator in EPC-mediated neovascularization. To explore the function of HDAC7 in neovascularization induced by EPCs, we use microarrays to profile the genes expression changes in HDAC7 lacking EPCs. HDAC7 silencing upregulated antiangiogenic genes transcription and suppressed proangiogenic genes expression, turning off the angiogenic switch for vessel formation. EPCs were isolated from human umbilical cord blood of healthy volunteers and the 3rd passage of cultured EPCs were selected for lentivirus infection. EPCs isolated from the same volunteer were devided into KD and NC group. EPCs were infected with lentivirus carrying HDAC7 shRNA in KD group and nonspecific random shRNA in NC group. Each group contains 2 samples from different volunteers for RNA extraction and hybridization on Affymetrix microarrays.