RNA-seq analysis lncRNA and mRNA between crushed sciatic nerves and intact contralateral nerves

To investigate the potential function of lncRNAs in Schwann cell (SCs) response to sciatic nerve damage and repair, we extracted the total RNA of crushed sciatic nerves and intact contralateral nerves for RNA-sequencing (RNA-seq). A total of 98 differentially expressed lncRNAs (including 46 up-regulated and 52 down-regulated lncRNAs) and 77 differentially expressed mRNAs (including 55 up-regulated and 22 down-regulated mRNAs) were identified between crushed sciatic nerves and normal control (log 2 FC > 1 and p < 0.001). Overall design: Nerve lesions were produced on the right side and the intact contralateral nerve served as the uninjured control. At the appropriate time points, total RNA of crushed sciatic nerves and intact contralateral nerves were extracted. RNA-seq data from 3 biological replicates was collected.