Supp-Hcun-LSM

The fall webworm (<i>Hyphantria cunea</i>), a typical destructive invasive pest, has caused substantial damage to both the ecological environment and economy in China. It primarily utilizes its antennae to locate food and perceive pheromones when external odor molecules stimulate the olfactory receptor neurons on the antennae, which then project their axons to the olfactory glomeruli within the antennal lobes where the olfactory information is integrated. However, the precise tissue structure of <i>H. cunea</i> antennal lobes remains unclear.To decipher the primary mechanism behind olfactory recognition in <i>H.cunea</i>, our study employed synaptic antibody immunostaining, as well as cluster staining of olfactory sensory neurons (OSNs), and computer-based reconstruction to establish a three-dimensional olfactory glomerular map of the moth's antennal lobes. A total of 74 male and 81 female olfactory glomeruli were identified, including 3 male-specific glomeruli (Macroglomerular complex, MGC) and 8 female-specific glomeruli (DL1-DL8). While the Cumulus (Cu) volume was largest in MGC, the differences in volume among DM-A and DM-P were minimal. These findings lay the groundwork for a better understanding of the unique olfactory perception mechanism in <i>H.cunea</i>.