Amplicons for NGS were prepared following cloning of PCR fragments into mammalian expression vectors as outlined in Fig 8A. The average number of full-length reads was determined based on the percenta
aThe total volume of PCR reaction was 25 µl and the PCR conditions were as follows: 50°C for 2 min, 95°C for 10 min, followed by 40 cycles of 95°C, 15 s for denaturation and 60°C, 1 min for annealing
