Text S1 - Lifespan Differences in Hematopoietic Stem Cells are Due to Imperfect Repair and Unstable Mean-Reversion

It is first shown that the empirical estimator of the time-to-failure, or lifespan, of the repopulation capacity of clonal long-term repopulating hematopoietic stem cells is statistically unbiased, and almost efficient relative to a Gumbel distribution of maximum extremes. Together, these properties indicate that the Gumbel distribution makes best use of the information obtainable from serial repopulation experiments. Table S1 contains experimentally determined HSC lifespans, growth and decline rates, and the Hurst exponents calculated using Algorithm 2 (compare Materials & Methods). Figure S1 shows the shapes of clonal repopulation time series obtained experimentally by measuring the percent donor-type cells in blood after transplanting a single HSC. (PDF)本研究首先证实，克隆性长期重建造血干细胞（clonal long-term repopulating hematopoietic stem cells）的重建造血能力的失效时间（即寿命）的经验估计量具有统计无偏性，且相较于极大值耿贝尔分布（Gumbel distribution of maximum extremes）近似有效。上述两项特性表明，耿贝尔分布能够最优利用连续重建造血实验可获取的全部信息。附表S1收录了实验测定的造血干细胞（Hematopoietic Stem Cell, HSC）寿命、增殖与衰减速率，以及通过算法2（参见材料与方法）计算得到的赫斯特指数。附图S1展示了通过移植单个造血干细胞后检测血液中供体型细胞占比所获得的实验性克隆重建造血时间序列的形态。（PDF）