Deletion of an sRNA primes development in a multicellular bacterium

Small non-coding RNAs (sRNAs) are essential in regulating gene expression during many biological processes. The myxobacteria gene pxr encodes an sRNA known to block fruiting-body development, an aggregative multicellular process triggered by starvation. Deletion of pxr allows Myxococcus xanthus cells to develop in the presence of nutrients. However, potential Pxr binding targets and most genes regulated by Pxr remain unknown. Here, we found that the absence of pxr expression dramatically alters the temporal dynamics of development, thus suggesting an important new role of this sRNA in myxobacterial ecology. We transcriptionally profiled vegetative cells of M. xanthus strains possessing vs lacking pxr and found that over half of the genes impacted by pxr deletion during growth are linked to development, including known and potentially novel critical regulators. Many other genes are associated with general metabolic processes, which Pxr regulates positively. Our study discovers new phenot...,
RNA sequencing
Timelapse microscopy
Bacteria cell culture
Western blot analysis for protein detection
, , # Deletion of an sRNA primes development in a multicellular bacteria.

[https://doi.org/10.5061/dryad.xsj3tx9pn](https://doi.org/10.5061/dryad.xsj3tx9pn)

**The Supplementary Information consist of:**

* 6 **Supplementary Tables** - Supplementary Tables 1 to 6 in .xlsx format.
* 7 **Supplementary Network Images** - PDF files reporting all networks discussed in the study with a greater resolution.
* 3 **Supplementary Data** - 1 file in .xlsx format (Supp. Data 1. Developmental time WT-deltaPxr-deltaPxrA.xlsx), 1 file in .csv format (Supp. Data 2. M. xanthus gene annotation from UniProt.csv), and 1 image in .pdf format (Supp. Data 3. Original images of the blot membrane). These data are directly relevant to results present in the main figures.
* 1 **Supplementary Code** - folder containing 1 R script (app.R), two data frames in .xlsx format (data.xlsx and dev_data.xlsx)
* **Supplementary Figure Raw Data** - 2 files in .xlsx format (Supp. Fig. 1a and Supp. Fig. 1b). These file are relative...

小型非编码RNA（small non-coding RNAs, sRNAs）在众多生物学过程的基因表达调控中发挥不可或缺的作用。粘球菌基因pxr编码一种已知可阻断子实体发育的sRNA——子实体发育是一种由饥饿触发的聚集型多细胞过程。缺失pxr可使黄色粘球菌（Myxococcus xanthus）细胞在营养充足的条件下完成发育进程。然而，目前Pxr的潜在结合靶点以及受其调控的绝大多数基因仍未明确。本研究发现，pxr表达缺失会显著改变发育过程的时序动态，这表明该sRNA在粘球菌生态中具有此前未被认知的重要新功能。我们对携带与缺失pxr的黄色粘球菌营养体细胞进行了转录组分析，发现生长过程中受pxr缺失影响的基因中超过一半与发育过程相关，包括已知的以及潜在的新型关键调控因子。另有众多其他基因与Pxr正向调控的一般代谢过程相关。本研究揭示了全新的表型……
RNA测序、延时显微成像、细菌细胞培养、用于蛋白检测的蛋白质免疫印迹（Western blot）分析

# 缺失一种非编码RNA可触发多细胞细菌的发育程序

https://doi.org/10.5061/dryad.xsj3tx9pn

**补充信息包含：**
* 6 **补充表格**——即1至6号补充表格，格式为.xlsx。
* 7 **补充网络图谱**——PDF格式文件，包含本研究中讨论的所有网络的高分辨率版本。
* 3 **补充数据集**——1份.xlsx格式文件（补充数据集1：发育时序WT-Δpxr-ΔpxrA.xlsx）、1份.csv格式文件（补充数据集2：来自UniProt的黄色粘球菌基因注释.csv）以及1份.pdf格式图像文件（补充数据集3：印迹膜原始图像）。上述数据与主图呈现的结果直接相关。
* 1 **补充代码**——包含1个R脚本（app.R）、2份.xlsx格式数据框（data.xlsx与dev_data.xlsx）的文件夹。
* **补充图片原始数据**——2份.xlsx格式文件（补充图1a与补充图1b）。这些文件对应……