A 3D Adult Zebrafish Brain Atlas (AZBA) for the Digital Age

Zebrafish have made significant contributions to our understanding of the vertebrate brain and the neural basis of behavior, earning a place as one of the most widely used model organisms in neuroscience. Their appeal arises from the marriage of low cost, early life transparency, and ease of genetic manipulation with a behavioral repertoire that becomes more sophisticated as animals transition from larvae to adults. To further enhance the use of adult zebrafish, we created the first fully segmented three-dimensional digital adult zebrafish brain atlas (AZBA). AZBA was built by combining tissue clearing, light-sheet fluorescence microscopy, and three-dimensional image registration of nuclear and antibody stains. These images were used to guide segmentation of the atlas into over 200 neuroanatomical regions comprising the entirety of the adult zebrafish brain. As an open source, online (azba.wayne.edu), updatable digital resource, AZBA will significantly enhance the use of adult zebrafish...

斑马鱼（Zebrafish）在推动人类对脊椎动物大脑及行为神经基础的理解方面做出了重要贡献，已成为神经科学领域应用最为广泛的模式生物之一。其作为研究模型的优势，在于兼具低成本、幼体阶段通体透明、易于进行遗传操作，且随个体从幼体向成体过渡时行为谱愈发复杂的多重特点。为进一步推动成年斑马鱼的研究应用，我们构建了首个全分段三维数字化成年斑马鱼脑图谱（AZBA，Adult Zebrafish Brain Atlas）。该图谱通过整合组织透明化技术、光片荧光显微镜（Light-sheet fluorescence microscopy）成像，以及细胞核与抗体染色的三维图像配准流程构建而成。利用这些成像结果，我们将该图谱分割为覆盖成年斑马鱼全脑的200余个神经解剖区域。作为一款开源、可在线访问（网址：azba.wayne.edu）且支持更新的数字化资源，AZBA将显著提升成年斑马鱼的研究应用...