Comparative analysis of gut bacteriophage between older and young people

Given the gut microbiota involve aging processing, we performed comparative analysis of gut bacteriophage between older and young subjects using next-generation sequencing (NGS). In our previous study, we found that the Ruminococcaceae is higher in aged subjects comparing to young one. To identify the bacteriophage targeting to the Ruminococcaceae, we also access the composition of phage in the Ruminococcaceae (ATCC, TSD-27) after incubated with human stool samples. The Lactobacillus (ATCC, LGG) targeting phage was used as the control. The virome sequencing analysis using NGS indicated that Myoviridae are high enrich in young subjects and predominate in TSD-27 targeting phage. Overall design: The study involved 24 healthy volunteers divided into a young group (25.6 ± 4.2 years old, n=12) and an older group (65.3 ± 5.6 years old, n=12) by age. The participants in the two groups were matched for age and gender. Six of samples was mixed as one group for DNA sequencing.