RNA-seq profiling of 368T1 murine KP NSCLC cells deleted for AMPK with AMPKa1 add-back subjected to no glucose conditions, Replicate 2

This dataset was generated for the paper, "Genetic analysis reveals AMPK is required to support tumor growth in murine Kras-dependent lung cancer models." Murine Kras mutant, p53 null (KP) 368T1 NSCLC cells were deleted for AMPK using the CRISPR/Cas9 system and subsequently stably infected with control vector ("KO") or AMPKalpha1 cDNA ("A1") add-back. These cells were subjected to no glucose (0mM, "NG") conditions for 12 or 18 hours, and profiled by RNA-sequencing. Replicate 2 High Glucose (HG) and No Glucose (NG) conditions were generated simultaneously and analyzed together. Analysis of a single dataset was performed using Replicate 1 ("R1"), and both replicates ("R1" and "R2") were analyzed together as indicated.

本数据集为论文《遗传分析揭示AMPK在小鼠Kras依赖性肺癌模型中支持肿瘤生长所必需》所构建。研究人员采用CRISPR/Cas9系统对小鼠Kras突变型、p53缺失型(KP)368T1非小细胞肺癌(Non-Small Cell Lung Cancer, NSCLC)细胞进行AMPK基因敲除，随后分别以对照载体（标记为"KO"）或AMPKα1互补DNA（cDNA，标记为"A1"）进行稳定感染以实现基因回补。将上述细胞置于无糖（0mM，标记为"NG"）培养条件下分别培养12小时与18小时，随后通过RNA测序(RNA-sequencing)开展转录组谱学分析。同步构建了重复2组的高糖（HG）与无糖（NG）培养样本，并将其与其余样本联合分析。单数据集分析仅采用重复1（R1）的数据；而如前文所述，针对两份重复样本（R1与R2）的联合分析则按指定方式开展。