Western blot HADHA & HADHB deficient fibroblasts and controls

Mitochondrial trifunctional protein (TFP) deficiency fibroblasts were submitted to sonication cell lysis. Western blots were performed with these cell lysates employing polyclonal anti-total TFP, monoclonal anti-HADHA, and monoclonal anti-HADHB antibodies, having TOMM-20 as a control.将线粒体三功能蛋白（Mitochondrial trifunctional protein, TFP）缺陷型成纤维细胞进行超声细胞裂解处理。以所得细胞裂解液开展蛋白质免疫印迹实验，使用多克隆抗总TFP抗体、单克隆抗HADHA抗体及单克隆抗HADHB抗体作为检测抗体，并以TOMM-20作为内参对照。