Data for the research article "High-resolution reconstruction of pH upregulation and its seasonal drivers in the temperate coral Cladocora caespitosa"

This repository contains the coral calcification and geochemical data files used to generate the results for the following article: MJ. Vergotti, DK. Kersting, TC. Brachert, S. Comeau, DA. Frick, EC. Hathorne, MJ. Henehan, J. Holtz, N. Teixidó, V. van Schijndel, JP. D'olivo (in prep.) High-resolution reconstruction of pH upregulation and its seasonal drivers in the temperate coral Cladocora caespitosa. Abstract: Ocean acidification (OA) and associated changes in seawater carbonate chemistry, combined with thermal stress, hampers coral calcification. By upregulating pH and dissolved inorganic carbon, corals can optimize their calcification, giving them some resilience to OA. Little is known about the seasonal- and interannual scale impacts of thermal stress and OA on pH upregulation and calcification in the temperate coral Cladocora caespitosa, despite it being the only zooxanthellate reef builder in the Mediterranean Sea. δ¹¹B and B/Ca were determined seasonally in C. caespitosa skeletons from two NW Mediterranean sites to reconstruct the effect of seawater temperature and pH on the carbonate chemistry of the coral calcifying fluid (CF), at a bimonthly resolution from June 2013 to August 2017 (Columbretes Islands, Spain), and June 2016 to February 2022 (Villefranche-sur-Mer, France). Cladocora caespitosa displayed a similar pH upregulation strategy to most tropical corals, albeit with an apparently lower sensitivity to seasonal environmental change. Temperature was the main driver of seasonal variability in the CF composition and coral calcification, with seawater pH having a comparatively lower seasonal variability, and acting on longer timescales. While longer coral records and investigations into inter-population variability would still be beneficial in order to fully understand the response of C. caespitosa to environmental change, our records constitute an important first step in understanding the biomineralization strategy of this ecologically important coral species. Funding and acknowledgments: We thank the Secretaría General de Pesca (MAPA) and the Columbretes Islands Marine Reserve staff for sampling authorization and logistic support in the gathering of biological and environmental data. We thank Dr. J.E. Hoffmann for giving us access to the micro-mill. We thank Dr. B. Chen, Dr. S. Ring and A. Balduin for their support in the lab, and J. Bernebee-Sey for his assistance in processing the data. We thank G.O. Cardoso for insightful conversations and early manuscript readings and comments. This study was partially funded by the European Union’s Horizon 2020 program of research and innovation (under the MaCoBioS grant agreement no. 869710), and by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation, project no. 401447620).本数据集仓库包含用于生成下述论文成果的珊瑚钙化与地球化学数据文件。 MJ. Vergotti、DK. Kersting、TC. Brachert、S. Comeau、DA. Frick、EC. Hathorne、MJ. Henehan、J. Holtz、N. Teixidó、V. van Schijndel、JP. D'olivo（待刊）：《温带簇枝珊瑚（Cladocora caespitosa）中pH上调及其季节驱动因子的高分辨率重建》 摘要：海洋酸化（OA）与海水碳酸盐化学的相关变化叠加热胁迫，会抑制珊瑚的钙化作用。珊瑚可通过上调pH与溶解无机碳浓度优化自身钙化过程，从而获得一定的海洋酸化耐受能力。尽管温带簇枝珊瑚（Cladocora caespitosa）是地中海唯一的虫黄藻共生造礁珊瑚，但学界对热胁迫与海洋酸化在季节及年际尺度上对该物种pH上调过程与钙化作用的影响仍知之甚少。 研究人员对地中海西北部两个采样点的温带簇枝珊瑚（Cladocora caespitosa）骨骼进行了季节性的δ¹¹B与B/Ca比值测定，以重建海水温度与pH对珊瑚钙化液（CF）碳酸盐化学的影响，采样分辨率为双月一次，采样时段分别为2013年6月至2017年8月（西班牙科卢姆布雷特斯群岛）以及2016年6月至2022年2月（法国滨海自由城）。温带簇枝珊瑚（Cladocora caespitosa）的pH上调策略与多数热带珊瑚相似，但对季节环境变化的敏感性明显更低。温度是钙化液组成与珊瑚钙化作用季节变化的主要驱动因子，而海水pH的季节变化幅度相对较小，且其影响作用于更长的时间尺度。尽管若要全面理解温带簇枝珊瑚（Cladocora caespitosa）对环境变化的响应，仍需获取更长的珊瑚记录并开展种群间差异研究，但本研究的观测数据仍是理解这一具有重要生态价值的珊瑚物种生物矿化策略的重要起步。 资助与致谢：感谢西班牙渔业总秘书处（Secretaría General de Pesca, MAPA）与科卢姆布雷特斯群岛海洋保护区的工作人员，为生物与环境数据的采集提供采样许可与后勤支持。感谢J.E. Hoffmann博士允许我们使用微型研磨机。感谢B. Chen博士、S. Ring博士与A. Balduin在实验室工作中提供的协助，以及J. Bernebee-Sey在数据处理方面给予的帮助。感谢G.O. Cardoso与我们开展富有启发性的讨论，并对稿件早期版本进行审阅与提出评论。本研究部分由欧盟地平线2020研究与创新计划（MaCoBioS资助协议编号：869710）以及德国研究基金会（Deutsche Forschungsgemeinschaft, DFG，项目编号：401447620）资助。