Enhancer control of promoter activity and variability via frequency modulation of clustered transcriptional bursts
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The dataset contains the intensity tracks used in the paper: Enhancer control of promoter activity and variability via frequency modulation of clustered transcriptional bursts. The dataframe is composed as follows: unique_id: identifier for the track clone: name of the clone frame: frame number x: x coordinate of the spot y: y coordinate of the spot mean_spot: raw mean spot intensity mean_spot_bleach: mean spot intensity after bleaching correction corrected_intensity: mean spot intensity after removal of local background mean_localbackground: mean raw intensity of the local background mean_localbackground_bleach: mean intensity of the local background after bleaching correction mean_completecell: mean intensity of the cell spotdetected: spot detection using Trackpy, subsequent filter, and removal of single bursts (burst of 1 frame)
本数据集涵盖论文《增强子通过调控簇状转录爆发的频率以控制启动子活性与变异性》(Enhancer control of promoter activity and variability via frequency modulation of clustered transcriptional bursts)中所使用的强度轨迹。 该数据框的组成字段如下: unique_id: 轨迹唯一标识符 clone: 克隆株名称 frame: 成像帧数 x: 靶点的x轴坐标 y: 靶点的y轴坐标 mean_spot: 靶点原始平均强度 mean_spot_bleach: 经光漂白校正后的靶点平均强度 corrected_intensity: 扣除局部背景后的靶点平均强度 mean_localbackground: 局部背景的原始平均强度 mean_localbackground_bleach: 经光漂白校正后的局部背景平均强度 mean_completecell: 完整细胞的平均强度 spotdetected: 经Trackpy完成靶点检测、后续筛选并移除单帧转录爆发(仅含1帧的爆发事件)后的检测结果



