Details of the proteins identified in Brassica napus resistant Charlton and susceptible RQ001-02M2 at various times after inoculation with Sclerotinia sclerotiorum.

ASpot numbers as given on the 2-D gel image (Figure 3) that were significantly affected in response to the pathogen challenge.Bhpi = hours post inoculation.CExpression ratios (fold changes) for each protein was calculated from the average of spot intensities values of treatment with respect to their control genotype at each time point. However, spots 30–37 represent the actual values of the spot density data in the absence of detection of any protein in the control genotype (for these spots). These spot densities were measured from the filtered 2-DE images and each spot density value comprises the sum of the signal intensities (expressed as spot/optical density units) of all the pixels that make up the object.DSE represents the standard error associated with the mean value of expression ratio for each spot and for each genotype at different time points separately.二维凝胶（2-D gel）图像（图3）中标注的蛋白斑点编号，对应在病原体侵染刺激后出现显著表达变化的斑点。Bhpi指代接种后小时数（hours post inoculation）。各蛋白的表达比值（即倍数变化，fold changes）通过计算各时间点处理组与对应对照基因型的斑点平均信号强度之比得到。但斑点30~37的数据为实际斑点密度值，此类斑点在对照基因型中未检测到任何蛋白。上述斑点密度通过过滤后的二维凝胶电泳（2-DE）图像测得，每个斑点的密度值为构成该斑点的所有像素的信号强度之和，单位为斑点/光密度单位。DSE代表每个斑点、每个基因型在各时间点的表达比值均值分别对应的标准误（standard error）。