Nanostructured Hydroxyapatite from Hen´s Eggshells Using Sucrose as a Template

Abstract The nanostructured hydroxyapatite (HAp) was synthesized by precipitation from aqueous phase, using hen’s eggshells and different sucrose concentrations. The XRD results confirmed the formation of HAp, with crystallite size of about 15-22 nm. The SEM and TEM characterization showed morphological changes when increasing the amount of sucrose used, with the tendency to form spheroidal particles. The specific surface area increases from 36 to 93 m2.g-1 as the amount of sucrose used increases. Our results indicated that sucrose can be used as a promising additive/template, easy to obtain at low cost. In addition to contributing to the recycling of this biowaste (eggshells), the synthesized HAp has the potential to be used in bone tissue engineering applications, since the samples were no cytotoxicity to dental pulp stem cells in the in vitro assessment by resazurin assay.摘要 本研究以鸡蛋壳为原料，通过水相沉淀法合成了纳米结构羟基磷灰石（hydroxyapatite, HAp），并辅以不同浓度的蔗糖。X射线衍射（X-ray diffraction, XRD）结果证实了羟基磷灰石的成功合成，其晶粒尺寸约为15~22 nm。扫描电子显微镜（scanning electron microscope, SEM）与透射电子显微镜（transmission electron microscope, TEM）表征结果显示，随着蔗糖添加量的提升，样品形貌发生演变，呈现出形成球状颗粒的趋势。随着蔗糖添加量增加，材料的比表面积从36 m²·g⁻¹提升至93 m²·g⁻¹。本研究结果表明，蔗糖可作为一种极具应用前景的添加剂/模板剂，且易于获取、成本低廉。该合成工艺不仅实现了鸡蛋壳这一生物废弃物的回收利用，所合成的羟基磷灰石还具备骨组织工程应用潜力；通过刃天青（resazurin）法开展的体外细胞毒性评估显示，所制备的样品对牙髓干细胞（dental pulp stem cells）无细胞毒性。